Multiplex editing

Extracted Attributes

• Primary Tools

  CRISPR-Cas9, MAGE (Multiplex Automated Genome Engineering)

• Core Advantage

  Simultaneous editing of multiple genomic sites to address complex traits driven by gene networks

• Key Applications

  Agriculture (crop yield enhancement), Medicine (CAR-T cell therapy), Biofuels

• Scientific Field

  Genetics and Biotechnology

• Complexity Factor

  Requires extensive knowledge of gene networks; for example, the wheat genome is five times larger than the human genome

Timeline

• Harris Wang and George Church develop Multiplex Automated Genome Engineering (MAGE) at the Wyss Institute at Harvard Medical School. (Source: Web search: Multiplex Automated Genome Engineering (MAGE))

  2009-01-01

• Inari Agriculture releases a whitepaper detailing the necessity of multiplex gene editing for tackling complex agricultural challenges. (Source: Web search: [PDF] Multiplex Gene Editing - Inari Agriculture)

  2023-01-01

• David Friedberg announces he is stepping down from investing to lead a stealth gene-editing company focused on multiplex editing for agriculture. (Source: Document 28121141-2767-4e7a-8206-a64e02347ba7)

  2023-12-01

• Publication of research on orthogonal CRISPR systems for multiplex base editing to enable nonviral engineering of allogeneic CAR-T cells. (Source: Web search: Orthogonal CRISPR systems for targeted integration and multiplex...)

  2025-12-03

Multiplexing

In telecommunications and computer networking, multiplexing (sometimes contracted to muxing) is a method by which multiple analog or digital signals are combined into one signal over a shared medium. The aim is to share a scarce resource—a physical transmission medium. For example, in telecommunications, several telephone calls may be carried using one wire. Multiplexing originated in telegraphy in the 1870s, and is now widely applied in communications. In telephony, George Owen Squier is credited with the development of telephone carrier multiplexing in 1910. The multiplexed signal is transmitted over a communication channel such as a cable. The multiplexing divides the capacity of the communication channel into several logical channels, one for each message signal or data stream to be transferred. A reverse process, known as demultiplexing, extracts the original channels on the receiver end. A device that performs the multiplexing is called a multiplexer (MUX), and a device that performs the reverse process is called a demultiplexer (DEMUX or DMX). Inverse multiplexing (IMUX) has the opposite aim as multiplexing, namely to break one data stream into several streams, transfer them simultaneously over several communication channels, and recreate the original data stream. In computing, I/O multiplexing can also be used to refer to the concept of processing multiple input/output events from a single event loop, with system calls like poll and select (Unix).

Web Search Results

• [PDF] Multiplex Gene Editing - Inari Agriculture

  If multiplex gene editing is the future, why aren’t major agricultural organizations focused on this technology? Multiplex gene editing is a new and complex approach. While several multinational and startup companies use genome editing tools for single-gene modifications, multiplex editing requires building the knowledge to determine exactly which genes and/or gene networks to simultaneously edit, currently an area of pioneering R&D. Does multiplex gene editing face any roadblocks? [...] 3 Multiplex gene editing is an advanced form of gene editing, which uses proven tools such as CRISPR to introduce natural modifications to the crop's DNA. These are modifications that would otherwise occur much more slowly over time and may have already been seen in a plant’s distant relative. This is similar to traditional breeding techniques but with far more precision in far less time and with far fewer resources. [...] Why Multiplex Gene Editing Matters Simple edits alone are not sufficient in tackling the biggest challenges we face. Plants’ genomes are incredibly complex. The wheat genome, for example, is five times bigger than the human genome. Meanwhile, characteristics such as water use efficiency are driven not by one or two native genes, but by a complex network in which many of these genes work together. Additionally, desired enhancements can require different types of edits. Sometimes a gene needs to be

• Multiplex Automated Genome Engineering (MAGE)

  Multiplex Automated Genome Engineering, or MAGE, is a genome editing technique that enables scientists to quickly edit an organism’s DNA to produce multiple changes across the genome. In 2009, two genetic researchers at the Wyss Institute at Harvard Medical School in Boston, Massachusetts, Harris Wang and George Church, developed the technology during a time when researchers could only edit one site in an organism’s genome at a time. Wang and Church called MAGE a form of accelerated evolution [...] because it creates different cells with many variations of the same original genome over multiple generations. MAGE made genome editing much faster, cheaper, and easier for genetic researchers to create organisms with novel functions that they can use for a variety of purposes, such as making chemicals and medicine, developing biofuels, or further studying and understanding the genes that can cause harmful mutations in humans. [...] Researchers were able to better understand how genomes worked as the access to sequencing technology increased, which enabled advanced genome editing technologies to emerge. Prior to MAGE, scientists commonly edited genomes using targetable nucleases, which are proteins that scientists can construct to recognize and cut specific sequences of DNA. Although that technology was precise and effective, it did not let engineers edit a genome in multiple places. Instead, they would have to introduce a

• Multiplexing - Wikipedia

  ### Frequency-division multiplexing [edit] [...] Cable TV has long carried multiplexed television channels, and late in the 20th century began offering the same services as telephone companies. IPTV also depends on multiplexing. ### Video processing [edit] Main article: Demultiplexer (media file) "Demultiplexer (media file)") In video editing and processing systems, multiplexing refers to the process of interleaving audio and video into one coherent data stream. [...] A variant technology, called wavelength-division multiplexing (WDM) is used in optical communications. ### Time-division multiplexing [edit]

• Multiplex CRISPR-Cas9 - Horizon Discovery

  A Cas9 stably expressing cell line will increase the efficiency of multiplexed editing, but may not be desired for cell line generation. Consider using either Cas9 mRNA or Cas9 protein to create a DNA-free system for cell line generation if your cells can be transfected or electroporated. [...] Each clonal cell line was sequenced for each individual edited gene using Sanger sequencing. Editing of genes was determined by deconvolution of the sequencing using the CRISP-ID tool. [...] ### Learn Blog Events News Quality eProcurement ### Need help? Customer & scientific support ## ### Languages English Japanese 1. Application Areas 2. Application Areas 3. CRISPR-Cas9 gene editing applications 4. Multiplex CRISPR-Cas9 # Multiplex CRISPR-Cas9 ## Knockout of more than one gene #### Edit multiple genes in a single reaction with synthetic crRNA

• Orthogonal CRISPR systems for targeted integration and multiplex ...

  REGNASE-1 with Streptococcus pyogenes Cas9 nucleases for targeted integration of an anti-CD19 CAR transgene at the T cell receptor α constant locus. Combined, these edits have been reported to generate safer allogeneic CAR-T cells with enhanced activity and persistence. We demonstrate multiplex gene editing in primary human T cells with B2M and REGNASE-1 base editing frequencies reaching 66% and 84%, respectively, while integrating the anti-CD19 CAR transgene in up to 36% or 71% of cells using [...] Multiple genomic modifications, including targeted transgene integrations and knockouts, may be required to develop potent, allogeneic chimeric antigen receptor (CAR)-T cell therapies. Conventional CRISPR-Cas systems generate double-strand breaks (DSBs) associated with genomic rearrangements and genotoxicities. DSB-free base editing reduces these risks. Here, we facilitate multiplex editing by combining Staphylococcus aureus Cas9 (SaCas9) mRNA base editors for DSB-free knockout of B2M and [...] Skip to article My account Sign in View PDF ## Molecular Therapy Volume 33, Issue 12, 3 December 2025, Pages 6082-6100 # Original Article Orthogonal CRISPR systems for targeted integration and multiplex base editing enable nonviral engineering of allogeneic CAR-T cells Author links open overlay panel, , , , , , , , rights and content Under a Creative Commons license Open access